脑清片作为一种地方性复方制剂,长期以来在我国山东、河北、东北等地区被广泛应用。虽然文献曾报道利用双波长分光光度法测定该药两种主要成分的含量,但其所含的咖啡因和氨基比林两种主要成分的含量测定在常规检验中却一直沿用着各地方药品标准中收载的容量分析方法。其中,氨基比林采用中和滴定,存在着由辅料干扰引起的空白和样品滴定终点色泽不一致的误差;咖啡因采用氯仿萃取后的剩余碘量法,操作繁琐、速度慢、误差大。鉴于上述情况,也鉴于近年来HPLC法的广泛应用和普及;同时,考虑到正相高效液相色谱 Brain clear piece as a local compound preparation, for a long time in our country Shandong, Hebei, northeast China is widely used. Although the literature has reported the use of dual-wavelength spectrophotometric determination of the two main components of the drug content, but its content of caffeine and aminopyrine contained two main components in the routine test has been used in all local drug standards In the collection of capacity analysis. Among them, aminopyrine with neutralization titration, there is interference caused by the blank and sample titration endpoint color inconsistencies error; caffeine by chloroform residual iodine method after extraction, cumbersome operation, slow, the error is large. In view of the above, but also in view of the widespread use and popularity of HPLC methods in recent years; the same time, taking into account the normal phase high performance liquid chromatography