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为了解我国成都汉族群体和大理白族群体 D3 S1545 基因座的遗传多态性,获得中国汉族和大理白族 D3 S1545 基因座的群体遗传学数据。采集了 148 名无血缘关系的中国成都汉族个体和96 名无血缘关系的大理白族个体的 E D T A 抗凝血样。 Chelex 法提取 D N A。 P C R 扩增样本 D N A,聚丙烯酰胺凝胶水平电泳分型。结果显示:在中国成都汉族群体中, D3 S1545 共发现 9 个等位基因,等位基因频率为 D3 S15458:00642; D3 S15459:00034; D3 S154510:0.0068; D3 S154511:0.0203; D3 S154512:0.1824; D3 S154513:0.3075; D3 S154514:03243; D3 S154515:0.0844; D3 S154516:0.0068。在大理白族群体中, D3 S1545 共发现6 个等位基因,等位基因频率为 D3 S15458:0.00781; D3 S154511:0.0261; D3 S154512:0.1719; D3 S154513:0.3906; D3 S154514:02448; D3 S15
To understand the genetic polymorphism of D3 S1545 locus in Chengdu Han population and Dali Bai population in China, population genetic data of D3 S1545 locus in Han and Dali of China were obtained. A total of 148 unrelated Han Chinese individuals from Chengdu, China and 96 non-related Dali Bai individuals were enrolled in this study. Chelex method to extract D N A. P C R amplified sample D N A, polyacrylamide gel electrophoresis typing. The results showed that there were 9 alleles at D3 S1545 in Han Chinese population in Chengdu, China. The allele frequency was D3 S1545 * 8: 00642; D3 S1545 * 9: 00034; D3 S1545 * 10: 0. D3 S1545 * 12: 0.1824; D3 S1545 * 13: 0.3075; D3 S1545 * 14: 03243; D3 S1545 * 15: 0.0844; D3 S1545 * 16: 0.0068. In Dali Bai population, D3 S1545 found a total of six alleles, the allele frequency of D3 S1545 * 8: 0.00781; D3 S1545 * 11: 0.0261; D3 S1545 * 12: 0.1719; D3 S1545 13: 0.3906; D3 S154514: 02448; D3 S15