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应用古ＤＮＡ研究技术从５种海马药材中提取ＤＮＡ，用ＰＣＲ技术扩增约４５０ｂｐ的１２ＳｒＲＮＡ基因片段和约４９０ｂｐ的细胞色素ｂ基因片段。对扩增产物进行了限制性片段长度多态性（ＲＦＬＰ）分析和ＤＮＡ序列分析。用ＲＦＬＰ分析方法可以鉴别２种海马，用ＤＮＡ序列分析方法得到的分子遗传标记可以鉴别所有５种海马。对其它动物类药材的鉴定有一定参考价值。
DNA was extracted from five kinds of hippocampus using ancient DNA research techniques, and a PCR amplification of about 450 bp of 12S rRNA gene fragment and about 490 bp of cytochrome b gene fragment was performed. The amplified products were subjected to restriction fragment length polymorphism (RFLP) analysis and DNA sequence analysis. Two kinds of hippocampus can be identified by RFLP analysis, and all five kinds of hippocampus can be identified by molecular genetic markers obtained by DNA sequence analysis. Identification of other animal herbs have a certain reference value.