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目的 建立旁路激活补体引起渗出性胸膜炎的模型。方法 纯化的眼镜蛇毒因子 (CVF)胸膜腔内注射 ,一定时间后检测渗出液容积、总白细胞数、蛋白浓度和组胺浓度。预先给予各种抗炎药或同时给予眼镜蛇毒另一种毒性成分直接溶血因子 (DLF) ,观察对上述指标的影响。结果 CVF0 0 8～ 2mg·kg-1注入大白鼠胸膜腔能引起有明显量效关系和时效关系的渗出性炎症 ,表现和角叉菜胶 1mg·kg-1作用类似。苯海拉明和异丙肾上腺素都能显著减少上述模型的渗出液容积、总白细胞数、蛋白含量和组胺浓度 ;吲哚美辛、地塞米松和环磷酰胺虽不影响组胺释放 ,但也可减少渗出液容积、总白细胞数和蛋白含量 ;但细胞膜补体调节蛋白CD5 9对上述的渗出性炎症无明显影响 ;而DLF能增强CVF引起的渗出性炎症。结论 CVF胸膜腔内注射能引起对常用的抗炎药敏感的渗出性胸膜炎。其作用能被DLF增强
Objective To establish a model of bypass-activated complement causing exudative pleurisy. Methods Purified cobra venom factor (CVF) was injected intrapleurally and volume of exudate, total leukocytes, protein concentration and histamine concentration were measured after a certain period of time. Pre-given a variety of anti-inflammatory drugs or cobra venom given another toxic components of direct hemolytic factor (DLF), to observe the impact of these indicators. Results Intrathecal injection of CVF0 08 ~ 2 mg · kg-1 into the pleural cavity of rats induced exudative inflammation with a dose-effect relationship and time-dependence relationship, which was similar to that of carrageenan 1 mg · kg -1. Diphenhydramine and isoproterenol significantly reduced exudate volume, total leukocyte count, protein content and histamine concentration in the above model; although indomethacin, dexamethasone and cyclophosphamide did not affect histamine release, But also decreased exudate volume, total leukocyte count and protein content. However, CD5 9 had no significant effect on exudative inflammation mentioned above, whereas DLF increased CVF-induced exudative inflammation. Conclusion Intrapleural injection of CVF can cause exudative pleurisy sensitive to commonly used anti-inflammatory drugs. Its role can be enhanced by DLF