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目的:采用气相色谱法同时检测中药萝芙木中残留的11种农药。方法:采用GC-ECD,以OV-1701弹性石英毛细管柱为色谱柱(30 m×0.32 mm,0.45μm),载气为高纯氮气,程序升温方式对萝芙木不同产地、不同植株部位中11种农药残留进行分析,样品用正己烷-丙酮-水(3:6:1)混合溶剂浸泡,超声提取后萃取,滤液经层析柱萃取、净化。结果:11种农药残留在26min内均可很好的分离,各峰面积与内标的峰面积比值与浓度呈良好的线性关系(r≥0.997),11种农药的回收率范围为81.0%～107.5%。结论:该法操作简便、准确,灵敏度高,精密度良好,是一种检测多种农药残留的较好方法。
OBJECTIVE: To simultaneously detect 11 kinds of pesticide residues in traditional Chinese medicine Rauvolfia by gas chromatography. Methods: The chromatographic column (30 m × 0.32 mm, 0.45 μm) on OV-1701 elastic quartz capillary column was used as the carrier gas. High purity nitrogen was used as the carrier gas in the GC-ECD. Eleven kinds of pesticide residues were analyzed. The samples were soaked in a mixed solvent of n-hexane, acetone and water (3: 6: 1), extracted by ultrasonic and extracted. The filtrate was purified by column chromatography. Results: The 11 pesticide residues were well separated within 26 min. The peak area ratio of each peak area to internal standard showed a good linear relationship with the concentration (r ≥ 0.997). The recoveries of 11 pesticides ranged from 81.0% to 107.5 %. Conclusion: The method is simple, accurate, sensitive and precise. It is a better method to detect multiple pesticide residues.