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对从中间偃麦草与普通小麦品种烟农 15杂交后代(BC3F6 )中选育的双体异附加系山农Line15的形态学、白粉病抗性、细胞学、基因组荧光原位杂交(GISH)及RAPD进行鉴定分析。结果表明它的主要形态性状介于双亲之间;白粉病抗性鉴定结果表明山农Line15对白粉病高抗近免疫;根尖细胞染色体数目为 2n=44,PMCMⅠ染色体构型为 2n=22Ⅱ;以中间偃麦草总基因组DNA为探针的基因组荧光原位杂交(GISH),结果表明山农Line15是在小麦的遗传背景中附加了 2条中间偃麦草染色体,为小麦-中间偃麦草双体异附加系;遗传分析表明山农Line15的抗白粉病基因基本上可以确定来源于中间偃麦草的染色体;RAPD分析表明:在供试的 120个随机引物中有 1个引物S170 (- 5′- ACAACGCGAG -3′- )能在山农Line15中稳定地扩增出特异带型,可以作为山农Line15所附加的中间偃麦草染色体的特异分子标记。
Morphological, powdery mildew resistance, cytology, genomic fluorescence in situ hybridization (GISH) and RAPD markers were used to screen Line15 isolates from Thinopyrum intermedium and common wheat cultivar Yannong15. Identification analysis. The results showed that its main morphological trait was between parents. The results of powdery mildew resistance showed that Shannong Line15 was highly resistant to powdery mildew. The number of chromosomes in root tip cells was 2n = 44 and that of PMCMⅠ was 2n = 22Ⅱ. The results of genome-based fluorescence in situ hybridization (GISH) using the total genomic DNA of T. triticina as a probe showed that Shannong Line15 was added with two chromosomes from the genetic background of wheat to the wheat-Thinopyrum intermedium The results of RAPD analysis showed that there was one primer among the 120 random primers tested, S170 (- 5’-ACAACGCGAG -3’-) could stably amplify specific bands in Shannon Line15, which could be used as a specific molecular marker attached to the chromosome of Thinopyrum intermedium by Shannong Line15.