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体内扩散盒培养技术(Diffusion chamber)将细胞置于由微孔滤膜封闭的扩散盒内,再埋入经~(60)Co γ线致死量照射的昆明小鼠腹腔,可使细胞在比较接近自然的情况下生长。我们观察了4株小鼠白血病(L-615,L-7212,L-1210.P-388)细胞在扩散盒中生长的过程。培养6～7天,细胞经对数生长期后可达生长高峰,盒内细胞数可为植入时的5～14倍。如受鼠口(?)或肌注抗癌药物,可影响扩散盒内细胞的生长,其作用与肿瘤细胞对抗癌药物的敏感性有关。
Diffusion chamber The cells were placed in a diffusion chamber closed by a microporous membrane filter and then embedded into the peritoneal cavity of a Kunming mouse irradiated with ~ (60) Co γ radiation to allow the cells to be in close proximity Growing under natural conditions. We observed the growth of 4 murine leukemia (L-615, L-7212, L-1210.P-388) cells in a diffusion cassette. After cultured for 6 to 7 days, the cells reached the peak of growth after logarithmic growth phase, and the number of cells in the box can be 5 to 14 times of the time of implantation. Such as the rat mouth (?) Or intramuscular injection of anti-cancer drugs, can affect the proliferation of cells within the cell growth, its role and cancer cells on the sensitivity of anti-cancer drugs.