论文部分内容阅读
厌氧消化链球菌是临床常见病原菌,在其鉴定中已较多应用气-液相色谱法。作者等对鉴定用肉汤成份稍加改良。此MNT 培养基含0.5%胰蛋白酶,0.5%酵母浸液,0.2%L-正亮氨酸和0.1%L-酪氨酸。经此肉汤培养细菌后用气-液相色谱法检测代谢产物的挥发和非挥发性酸。试验菌来自ATCC者计有消化链球菌属中的5种,葡萄球菌2种,链球菌1种;来自CDC 的葡萄球菌链球菌参考试验室者计有金黄色葡萄球菌9株,表皮葡萄球菌5株,腐生葡萄球菌6株,无乳链球菌4株,血链球菌Ⅰ、Ⅱ各1株,突变链球菌4株,来自CDC 厌氧菌参考试验室者计厌氧胨链球菌10株,不解糖消化链球菌10株,吲哚胨链球菌3株,巨大消化链球菌7株,普氏消化
Anaerobic digestion of Streptococcus is a common clinical pathogen, in its identification has been more application of gas-liquid chromatography. The author of the broth ingredients for identification with a slight improvement. This MNT medium contains 0.5% trypsin, 0.5% yeast extract, 0.2% L-norleucine and 0.1% L-tyrosine. After this bacterium is cultured in the broth, the volatile and non-volatile acids of the metabolites are detected by gas-liquid chromatography. Five strains of Streptococcus spp., Two species of Staphylococcus and one species of Streptococcus were counted from ATCC. Staphylococcus aureus reference strain from CDC included 9 strains of Staphylococcus aureus and 5 strains of Staphylococcus epidermidis 6 strains of Staphylococcus aureus, 4 strains of Streptococcus agalactiae, 1 strain of Streptococcus sanguis, 1 strain of Streptococcus, 4 strains of Streptococcus mutans, 10 strains of anaerobic streptococcus from anaerobic reference laboratory of CDC, 10 strains of Streptococcus digestin, 3 strains of Streptococcus indole, 7 strains of Giant Peptostreptococcus, Platts digestion