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采用磷酸钙－ＤＮＡ共沉淀法，将ｐＲＥＰ－８－ＩＦＮ－表达质粒直接注射至小鼠腹腔，可成功地转染腹腔巨噬细胞（ＭΦ），并表达其基因产物，能有效地抑制肿瘤生长；再次基因转染，可获得增强的抗肿瘤效应。还发现转基因ＭΦ所分泌的激活因子能激活未转基因的ＭΦ；增加腹腔ＭΦ的数量；增强ＭΦ的杀瘤活性；并使ＭΦ分泌一定水平的ＮＯ、ＩＬ－１和ＴＮＦ等，进一步增强抗肿瘤作用。转基因ＭΦ及其表达产物可使脾细胞ＮＫ活性和内源性ＬＡＫ活性增强，提高机体抗肿瘤功能，为肿瘤的细胞因子基因治疗提供了一种简便而有效的抗肿瘤新途径。
The plasmid pREP-8-IFN- was directly injected into the peritoneal cavity of mice by calcium phosphate-DNA co-precipitation method, which could successfully transfect peritoneal macrophages (MΦ) and express its gene products, which can effectively inhibit tumor Growth; again gene transfection, can obtain enhanced anti-tumor effect. It was also found that the activator secreted by transgenic MΦ can activate non-transgenic MΦ, increase the number of peritoneal MΦ, enhance the tumoricidal activity of MΦ, and make MΦ secrete certain levels of NO, IL-1 and TNF to further enhance the antitumor effect . Transgenic MΦ and its expression product can enhance NK activity and endogenous LAK activity of spleen cells and improve the anti-tumor function of the organism. It provides a simple and effective new anti-tumor approach for gene therapy of tumor cytokines.