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目的:探讨白花蛇舌草对哮喘小鼠气道炎症的治疗作用及其作用机制。方法:把40只清洁级BABL/c小鼠,随机数字表法分为4组,每组10只,分别为生理盐水对照组、哮喘模型组、白花蛇舌草组、糖皮质激素组。在末次激发24 h后所有小鼠取左肺组织行HE染色和PAS染色,观察哮喘组肺组织炎症浸润和结构破坏情况。用酶联免疫吸附法(ELISA)观察小鼠支气管肺泡灌洗液(BALF)中细胞的总数和各种炎症细胞计数的变化以及白细胞介素-4(Interleukin 4,IL-4)、白细胞介素-5(Interleukin5,IL-5)、白细胞介素13(Interleukin13,IL-13)及干扰素-γ(Interferon-gamma,IFN-γ)含量;取右肺组织用Westernblot检测NF-κB p65的表达变化情况。结果:哮喘模型组小鼠与对照组相比较,BALF中的嗜酸粒细胞计数及IL-4、IL-5、IL-13水平均出现增高,而IFN-γ水平出现降低,且肺组织中的NF-κB p65表达水平显著高于对照组(P<0.05);白花蛇舌草组和糖皮质激素组小鼠与哮喘组小鼠相比炎症细胞计数、IL-4、IL-5、IL-13以及NF-κB p65表达水平均显著降低而IFN-γ水平则明显上升,且具有显著性差异(P<0.05)。结论:白花蛇舌草通过阻断NF-κB p65表达,下调哮喘小鼠BALF中IL-4、IL-5、IL-13水平,同时上调BALF中IFN-γ水平以及降低炎症细胞数量,从而抑制气道炎症。
Objective: To investigate the therapeutic effect of Hedyotic diffusa on airway inflammation in asthmatic mice and its mechanism. Methods: Forty clean BABL / c mice were randomly divided into 4 groups (n = 10): normal saline control group, asthma model group, HHD, glucocorticoid group. The left lungs of all mice were stained with HE and PAS at 24 h after the last challenge, and the infiltration and destruction of lung tissue in the asthma group were observed. The total number of cells in the bronchoalveolar lavage fluid (BALF) and the changes of various inflammatory cell counts were observed by enzyme-linked immunosorbent assay (ELISA) and the expressions of interleukin-4 (IL-4), interleukin 5, Interleukin 5 (IL-5), Interleukin 13 (IL-13) and Interferon-gamma (IFN-γ) were measured. The expression of NF-κB p65 in the right lung was detected by Western blot. Changes. Results: Compared with the control group, the number of eosinophils and the levels of IL-4, IL-5 and IL-13 in BALF and the level of IFN-γ in BALF of asthmatic model group were significantly decreased The expression of NF-κB p65 was significantly higher than that of the control group (P <0.05). Compared with the mice in the asthma group, the numbers of IL-4, IL-5 and IL-4 in the HHD and glucocorticoid groups -13 and NF-κB p65 expression levels were significantly reduced and IFN-γ levels were significantly increased, and with significant differences (P <0.05). Conclusion: Hedyotis diffusa can inhibit the expression of NF-κB p65, down-regulate the levels of IL-4, IL-5 and IL-13 in BALF, upregulate the level of IFN-γ in BALF and the number of inflammatory cells in BALF Airway inflammation.