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目的建立脊髓损伤所致自发痛动物模型。方法健康成年Wistar大鼠30只分为2组,用WADE法复制脊髓L2节段3 Ncm(G1组)和2 Ncm(G2组)损伤,分别在损伤前、后15 min在L1节段记录体感诱发电位,分别在损伤前、后4、8、12、16、24 h记录大鼠躯干部皮肤对轻压、轻触觉刺激反应和前足对弗莱毛轻压的抬足率等痛阈变化,并每日观察大鼠自发痛行为学的变化,持续10个月。结果2组动物均出现痛超敏和明显的自发痛现象,其中G1组的严重自发痛(自噬)的发生率为50%,接近人类脊髓损伤(spinal cord injury,SCI)所致的中枢性疼痛(central pain,CP)中自发痛的比例。结论用WADE法复制大鼠3 Ncm SCI自发痛的模型具有良好的可重复性,其自发痛症状与人类SCI的CP接近,为研究自发痛发生发展机制提供了实验条件。
Objective To establish a spontaneous pain model induced by spinal cord injury. Methods Thirty healthy adult Wistar rats were divided into two groups. The spinal cord segments of 3 Ncm (L2 group) and 2 Ncm (G2 group) of L2 segment were duplicated by WADE method. The body sensation was recorded in L1 segment 15 min before and 15 min after injury Evoked potentials were recorded before and 4, 8, 12, 16, 24 h after injury, respectively, the trunk of the skin of rats recorded mild pressure, tactile stimulation reaction and front foot on the Fulai Mao mild pressure lift lift and other pain threshold changes, The changes of spontaneous pain behavior in rats were observed daily for 10 months. Results Pain hypersensitivity and spontaneous pain were observed in both groups. The incidence of severe spontaneous pain (autophagy) in group G1 was 50%, which was close to the central pain caused by spinal cord injury (SCI) (central pain, CP) spontaneous pain in the ratio. CONCLUSION: The model of spontaneous pain of rat with 3 Ncm SCI was successfully replicated by WADE method. The spontaneous pain symptom was close to CP of human SCI, which provided the experimental conditions for studying the mechanism of spontaneous pain.