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为研究原发性肝内胆管细胞癌男性发病率高的原因 ,采用受体放射配基结合分析法 ,检测 2 3例手术病人肝内胆管细胞癌及其癌旁组织和 6例正常肝内胆管细胞的胞浆及胞核的雄性激素受体 (AR)含量。胞浆及胞核AR受体浓度分别为正常肝 0 .2 0～ 5 .3 0fmol/mg蛋白、1.91～ 6.5 0fmol/mg蛋白 ,肝内胆管癌为 5 .12～ 2 7.62fmol/mg蛋白和 10 .73～47 18fmol/mg蛋白 ,癌旁组织为 3 .18～ 14 .98fmol/mg蛋白和 5 .15～ 3 6.3 2fmol/mg蛋白。胞核和胞浆AR的平均浓度为癌瘤 >癌旁组织 >正常肝胆管组织 ,其差异有显著意义。肝内胆管细胞癌及癌旁组织的AR浓度与患者性别、年龄、酗酒、乙肝表面抗原、丙肝抗体、甲胎蛋白、病理类型、细胞分化程度及基础肝胆病变均无直接关系。提示我国肝内胆管细胞癌患者男性明显多于女性与肝内胆管癌表达AR可能有某种联系。
In order to study the reason of the high incidence of primary intrahepatic cholangiocarcinoma in men, the receptor radioligand binding assay was used to detect intrahepatic cholangiocarcinoma and para-cancerous tissues in 23 patients and 6 normal intrahepatic bile ducts Cytosolic and cytoplasmic male androgen receptor (AR) content. The concentrations of AR and AR in cytoplasm and nucleus were 0.20 ~ 5.03.0fmol / mg protein and 1.91 ~ 6.50fmol / mg protein, respectively. The intrahepatic cholangiocarcinomas were between 5.12 ~ 7.662fmol / mg protein and 10.73 ~ 47 18fmol / mg protein, paracancerous tissues were 3.18 ~ 14.98fmol / mg protein and 5.15 ~ 3 6.32fmol / mg protein. The average concentration of nuclear and cytoplasmic AR was significantly different between carcinomas> adjacent tissues> normal liver and biliary tissues. Intrahepatic cholangiocarcinoma and paracancerous tissue AR concentration and patient gender, age, alcoholism, hepatitis B surface antigen, hepatitis C antibody, alpha fetoprotein, pathological type, degree of cell differentiation and basal hepatobiliary lesions were not directly related. Prompted that the number of patients with intrahepatic cholangiocarcinoma in our country significantly more than women and intrahepatic cholangiocarcinoma AR may have some connection.