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目的观察低剂量伽玛刀照射对癫大鼠海马神经元超微结构的影响。方法建立大鼠青霉素局灶性癫动物模型,将58只SD大鼠分为对照组、癫模型组和伽玛刀照射组。对大鼠行伽玛刀照射,照射中心剂量24 Gy、周边剂量12 Gy、等剂量曲线50%,术后3 h～60 d取靶区海马,透射电镜观察并采用图像分析系统对线粒体形态进行计量分析。结果对照组细胞结构基本正常;癫模型组可见神经元细胞器明显空化,线粒体体密度、数密度、比表面和嵴膜密度较对照组明显减少(均P<0.05),线粒体平均体积和平均截面积较对照组明显增大(均P<0.05)。伽玛刀照射组早期线粒体的平均体积、平均截面积、数密度、比表面与对照组相比差异显著(均P<0.05),中期和晚期线粒体各项参数与对照组相比差异不显著。结论大鼠癫发作早期线粒体形态结构变化明显,低剂量伽玛刀照射对神经元修复起重要作用。
Objective To observe the effects of low dose gamma knife irradiation on the ultrastructure of hippocampal neurons in epileptic rats. Methods The animal model of penicillin-induced focal epilepsy in rats was established. Fifty-eight SD rats were divided into control group, epilepsy model group and gamma knife irradiation group. The rats were exposed to Gamma Knife with a central dose of 24 Gy, a peripheral dose of 12 Gy and an isodose curve of 50%. The target hippocampus was harvested from 3 h to 60 d after operation. The morphology of the mitochondria was observed by transmission electron microscopy Metrology analysis. Results The cell structure of the control group was basically normal. The neuron organelles were obviously cavitated in the epilepsy model group. The mitochondrial body density, number density, specific surface area and crest densities were significantly decreased (all P <0.05), and the average mitochondrial volume and average Compared with the control group, the cross-sectional area increased significantly (all P <0.05). Compared with the control group, the average volume, average cross-sectional area, number density and specific surface area of early mitochondria in Gamma Knife irradiation group were significantly different (all P <0.05). The mitochondrial parameters in mid-stage and late stage were not significantly different from those in control group. Conclusion The morphological changes of mitochondria in the early stage of epileptic seizures in rats are obvious. Low dose Gamma Knife irradiation plays an important role in the repair of neurons.