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目的从蛋白质水平观察死亡相关蛋白激酶(DAPK1)、人类白细胞抗原-Ⅰ(HLA-Ⅰ)、雌激素受体(ER)α基因表达水平与不同病理级别的宫颈病变的相关性。方法收集汉族妇女宫颈炎、宫颈上皮内瘤样病变(CIN)和宫颈鳞癌患者石蜡包埋组织标本115例,采用免疫组织化学方法鉴定蛋白表达水平。结果 DAPK-1、ERα、HLA-Ⅰ基因在宫颈炎/CINⅠ组织中阳性表达率分别为90%、76%、88%;在CINⅡ/Ⅲ和宫颈鳞癌中这些基因阳性表达率分别为53%、48%;45%、24%;67%、40%。在CINⅡ/Ⅲ和宫颈鳞癌中这些基因阳性表达率明显低于宫颈炎/CINⅠ,各组阳性表达率差异显著(P<0.05),随着宫颈病变病理变化的加重,基因蛋白阳性表达率逐渐降低。结论 DAPK1、HLA-Ⅰ、ERα基因表达阳性或缺失可能是CIN及宫颈鳞癌的重要生物学特征,与宫颈癌发生关系密切,这些基因的检测可能作为预测CIN和宫颈癌的重要生物指标。
Objective To observe the relationship between the levels of DAPK1, HLA-Ⅰ, and estrogen receptor (α) gene and the pathological grades of cervical lesions at the protein level. Methods 115 cases of cervicitis, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma were collected from Han Chinese women. Immunohistochemistry was used to identify the protein expression. Results The positive rates of DAPK-1, ERα and HLA-Ⅰ in cervicitis / CINⅠ tissues were 90%, 76% and 88%, respectively. The positive rates of these genes in CINⅡ / Ⅲ and cervical squamous cell carcinoma were 53% , 48%; 45%, 24%; 67%, 40%. The positive rate of these genes in CINⅡ / Ⅲ and cervical squamous cell carcinoma was significantly lower than that in cervicitis / CINⅠ (P <0.05). With the increase of pathological changes of cervical lesions, the positive rate of gene protein gradually increased reduce. Conclusion The positive or deletion of DAPK1, HLA-Ⅰ and ERα gene may be important biological features of cervical intraepithelial neoplasia and cervical squamous cell carcinoma, which are closely related to the occurrence of cervical cancer. The detection of these genes may be used as important biomarkers for predicting CIN and cervical cancer.