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目的:观察光化学法诱导脑梗死大鼠大脑皮层一氧化氮(NO)与核转录因子-κΒ(NFκ-Β)含量的变化,以及中药山茱萸提取物(主要成分为环烯醚萜苷)对其的影响。方法:大鼠预先灌胃给药7 d后,制作光化学致脑梗死模型,采用分光光度法检测脑组织中NO和一氧化氮合酶(NOS)的变化,免疫组织化学方法检测NF-κΒ阳性细胞数量的变化。结果:模型组与对照组相比,出现了明显的梗死灶,脑皮层NO含量与NOS活性增加,NF-κΒ阳性细胞数量增多。与模型组相比,山茱萸提取物能明显减小脑梗死面积,降低脑皮层NO含量和NOS活性,并显著减少NFκ-Β阳性细胞数量。结论:山茱萸环烯醚萜苷可通过影响脑内NO,NFκ-Β的水平发挥对脑梗死的治疗作用。
OBJECTIVE: To observe the changes of nitric oxide (NO) and nuclear factor-κΒ (NFκ-Β) contents in cerebral cortex of cerebral infarction rats induced by photochemical method, and to observe the effects of Hawthorn extract (mainly composed of iridoid glycosides) on them. Impact. METHODS: After 7 days of pre-administration in rats, a photochemical cerebral infarction model was established. Spectrophotometric method was used to detect the changes of NO and NOS in brain tissue. Immunohistochemistry was used to detect NF-κB positive. Changes in the number of cells. RESULTS: Compared with the control group, the model group showed obvious infarction, NO content and NOS activity in the cerebral cortex increased, and the number of NF-κΒ positive cells increased. Compared with the model group, hawthorn extract can significantly reduce cerebral infarct size, reduce NO content and NOS activity in cerebral cortex, and significantly reduce the number of NFκ-Β positive cells. Conclusion: Hawthorn iridoid glycosides can play an important role in the treatment of cerebral infarction by affecting the level of NO, NFκ-Β in brain.