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OBJECTIVE To explore the inhibition of ACHN cells via shRNA expression vector mediated cyclinE1 gene silencing.METHODS The shRNA targeting at cyclinE1 gene was designed and synthesized. By ligation, the fragment was inserted into pGenesil-1-U6 to construct the recombinant plasmid pGenesil-1- U6-cyclinE1. The identified recombinant plasmid was introduced into ACHN cells with lipofectamine 2000. The inhibition of cyclinE1 mRNA and protein expression were analyzed by RT-PCR and western-blotting. MTT method was used for observing cell proliferation and drawing growth curve. The cell cycle and ratios of apoptotic cell were assessed by flow cytometric detection. The ability of invasion and speed of cell migration were detected by transwell chamber invasive models and cell scratch method.RESULTS The inhibition of expression of cyclinE1 in ACHN cells mediated by recombinant vector (0.0933 ± 0.05) was significantly lower than that in the group of transfected with empty vector (0.8827 ± 0.04) and the control group (0.9021 ± 0.03) (P < 0.05). Flow cytometry showed that recombinant cells were blocked in the G1 phase and the apoptotic ratio was increased significantly (11.15 ± 4.00)% (P < 0.05). The curves of cell growth indicated that the proliferation of cell transfected with recombinant plasmid was inhibited significantly compared with that in control group (P