论文部分内容阅读
用Affymetrix小麦基因芯片研究了小麦成熟胚在MS+2,4-D(2mg·L-1)培养基上脱分化过程中不同时间点的基因表达变化,用NCBI、DATF和DRTF等生物信息学相关网站对基因表达信息进行处理,并针对WRKY家族转录因子相关基因的变化情况进行分析的结果表明:WRKY家族中有20个相关基因在脱分化过程中的不同时点至少发生一次上调或下调表达变化,变化幅度分别为2￣40倍和2￣16倍,其中WRKY6、WRKY18、WRKY33及其下游基因CA692019、CA660172、CA640435等与小麦成熟胚脱分化过程可能有密切关系。WRKY6在脱分化的全过程中一直呈下降趋势,推测2,4-D诱导可能导致成熟胚细胞的抗性下降,从而有利于脱分化的进行。WRKY33基因可能在脱分化初期的信号转导中有作用。不同物种的组织或器官脱分化过程中的转录因子基因表达有差异,说明其脱分化机制的多样性和复杂性。
The gene expression changes of wheat mature embryos at different time points during the dedifferentiation of MS + 2,4-D (2 mg · L-1) medium were studied with Affymetrix Wheat GeneChip. Bioinformatics such as NCBI, DATF and DRTF Relevant websites deal with the gene expression information and analyzed the changes of the WRKY family transcription factor related genes. The results showed that there were at least one up-regulated or down-regulated expression of 20 related genes in WRKY family at different time points during dedifferentiation The changes and changes ranged from 2 to 40 times and 2 to 16 times, respectively. WRKY6, WRKY18 and WRKY33 and their downstream genes CA692019, CA660172 and CA640435 may be closely related to the dedifferentiation of mature wheat embryos. WRKY6 has been declining in the whole process of dedifferentiation, suggesting that 2,4-D induction may lead to decreased resistance of mature blast cells, which is conducive to dedifferentiation. The WRKY33 gene may play an important role in signal transduction during dedifferentiation. Different species of tissue or organ dedifferentiation process of transcription factor gene expression differences, indicating its dedifferentiation mechanism diversity and complexity.