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目的:研究蛇床子素(Ost)对人乳腺癌细胞阿霉素耐药株多药耐药(MDR)的逆转作用及其机制。方法:采用MTT比色法测定药物的细胞毒性,高效液相-紫外检测法检测细胞内ADR浓度。结果:Ost对MCF-7及MCF-7/ADR细胞均有增殖抑制作用,IC50值分别为(58.1±2.3)μmol.L-1和(59±5)μmol.L-1;在5~15μmol.L-1范围内,Ost可以不同程度地增强ADR对MCF-7细胞杀伤作用,与ADR联合用药降低ADR对MCF-7/ADR的IC50值,显著增加MCF-7/ADR细胞内ADR的积累。结论:Ost对人乳腺癌细胞株MCF-7及其阿霉素耐药株MCF-7/ADR细胞均有增殖抑制作用,而且Ost通过明显增加MCF-7/ADR细胞内ADR的浓度,逆转其阿霉素耐药性。
Objective: To investigate the reversal effect of Ostin on the multidrug resistance (MDR) of human doxorubicin-resistant human breast cancer cells and its mechanism. Methods: MTT colorimetric assay cytotoxicity, high performance liquid chromatography - UV detection of intracellular ADR concentration. RESULTS: Ost had inhibitory effects on proliferation of MCF-7 and MCF-7 / ADR cells with IC50 values of (58.1 ± 2.3) μmol.L-1 and (59 ± 5) μmol.L- .L-1, Ost can enhance the killing effect of ADR on MCF-7 cells to varying degrees. Combined with ADR can reduce the IC50 of ADR to MCF-7 / ADR and increase the accumulation of ADR in MCF-7 / ADR cells . CONCLUSION: Ost can inhibit the proliferation of human breast cancer cell line MCF-7 and its doxorubicin-resistant MCF-7 / ADR cells, and Ost reverses its effect by increasing the ADR concentration in MCF-7 / ADR cells Adriamycin resistance.