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目的：在树（Ｔｕｐａｉａ）体内观察硫代反义寡脱氧核苷酸（Ｓ－ＡＳＯＤＮ）对ＨＤＶ复制、表达的抑制作用。方法：树同时接种ＨＢＶ阳性血清０．１ｍｌ、ＨＤＶ阳性血清０．３ｍｌ后，将１６只ＨＤＶ／ＨＢＶ感染成功的树随机分为两组，给药组８只树按每只每次经尾静脉注射Ｓ－ＡＳＯＤＮ３ｍｇ，隔日１次，共７次。对照组中２只注射等量生理盐水，另６只不作处理。注射后５、１０、１５、２５ｄ取血及肝组织采用免疫组化、斑点杂交及原位杂交法检测ＨＤＶＡｇ及ＨＤＶＲＮＡ。结果：于注射结束时（１５ｄ）给药组有７只树肝内ＨＤＶＡｇ及ＨＤＶＲ－ＮＡ转为阴性，对照组８只树中仅１只转为阴性。停止使用Ｓ－ＡＳＯＤＮ１０ｄ后，给药组２只阴转树肝内又可检出ＨＤＶＡｇ和ＨＤＶＲＮＡ，对照组仍有７只可检出。结论：Ｓ－ＡＳＯＤＮ在树体内能有效抑制ＨＤＶ复制及表达，但作用并不完全，可能与用药剂量不足，时间不够长及仅用硫代修饰尚不能直接导向靶基因有关
OBJECTIVE: To observe the inhibitory effect of S-ASODN on the replication and expression of HDV in Tupaia. Methods: Tree shrews were simultaneously inoculated with 0.1 ml of HBV-positive serum and 0.3 ml of HDV-positive serum. Sixteen tree shrews with HDV / HBV infection were randomly divided into two groups. Eight tree shrews The tail vein injection of S-ASODN3mg, every other day, a total of 7 times. Two of the control group were injected with the same amount of saline, and the other 6 were not treated. At 5, 10, 15, 25 days after injection, the blood and liver tissues were examined for HDVAg and HDVRNA by immunohistochemistry, dot blot hybridization and in situ hybridization. Results: HDVAg and HDVR-NA in the tree shrews turned negative at the end of injection (15d), and only one of the 8 tree shrews in the control group turned negative. After stopping the use of S-ASODN10d, HDVAg and HDVRNA were detected in the liver and liver in the 2 female tree in the administration group, and 7 in the control group were still detectable. CONCLUSIONS: S-ASODN can effectively inhibit the replication and expression of HDV in the shrews, but its effect is not complete, which may not be directly related to the target genes due to insufficient dosage, insufficient time and only thio-modification