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携带适合度优势基因的转基因作物如果长期存活于栽培系统之外,抗性基因可能转移到野生近缘种并带来生态问题.以转Osepsps基因水稻EP为材料,以非转基因受体“明恢86”为对照,通过田间定位试验,研究半野生生境下转Osepsps基因水稻的营养生长、繁殖能力以及稻田杂草的种类、频度和密度等指标变化,分析转Osepsps基因在半野生生境中的生态适合度以及对稻田杂草群落的影响.连续2年试验的结果显示:在常规栽培和半野生生境下,转基因稻与非转基因稻的株高、分蘖数、单株穗数、单株总粒数、单株实粒重和千粒重等指标没有显著差异;但结实率显著受环境影响,半野生生境下转基因水稻2年结实率为50.33%和17.72%,相比对照的68.82%和30.27%均显著下降(P<0.05);2012年转基因稻单株实粒数为335粒,显著少于非转基因对照的548粒(P<0.05).转基因稻与非转基因稻稻田的杂草种类均为9科9属9种;栽培稻田杂草密度和频度显著低于半野生稻田;相同生境下转基因稻与非转基因稻稻田杂草频度、密度总体上没有差异.本研究表明,与非转基因对照相比,转epsps基因稻生态适合度没有显著提高,在半野生生境下有所下降,其生态风险不高于栽培稻.
If long-term survival of genetically modified crops carrying fitness suitability genes outside the cultivation system, the resistance genes may be transferred to the wild relatives and bring about ecological problems.Taking Osepsps rice EP as material, non-transgenic recipient Hui 86 "as control. Through the field experiment, the changes of the index of nutrition growth, reproductive capacity, species, frequency and density of Osepsps transgenic rice in semi-wild habitat were studied. And the effect on the weed community in paddy field.The results of two consecutive years showed that under conventional and semi-wild habitats, the plant height, tiller number, panicle number per panicle, There was no significant difference in total grain number, grain weight per plant and grain weight per plant, but the seed setting rate was significantly affected by the environment. The 2-year seed setting rate of semi-wild transgenic rice was 50.33% and 17.72%, 68.82% compared with the control 30.27% (P <0.05). In 2012, the number of grain per plant of transgenic rice was 335, which was significantly less than that of non-transgenic control (548) (P <0.05) .The weed species of transgenic rice and non-transgenic rice all 9 families, 9 genera and 9 species. The density and frequency of weeds in cultivated rice fields were significantly lower than that in semi-wild rice fields. There was no difference in the frequency and density of weeds in transgenic rice and non-transgenic rice fields in the same habitat. Compared with the control, the ecological adaptability of transgenic rice epsps did not increase significantly, but decreased in semi-wild habitat, and its ecological risk was not higher than that of cultivated rice.