论文部分内容阅读
目的 观察腹腔注射胰岛素样生长因子 Ⅰ (IGF Ⅰ )对NOD鼠胰岛炎和糖尿病的发生情况以及其对胰岛Fas FasL表达的影响。方法 2 0只 4周龄NOD雌鼠随机分为 2组 ,每组 10只 ,实验组每周 2次腹腔注射 1mlIGF Ⅰ(40ng ml) ,对照组注射 1ml生理盐水 ,共 8周。实验动物在发生糖尿病 7天后或至 2 0周龄处死 ,观察血清胰岛素及C肽浓度、胰岛HE染色、免疫组织化学染色 检测胰岛Fas FasL的表达。结果 IGF Ⅰ可明显减少NOD鼠胰岛炎和糖尿病的发生 (P <0 .0 1) ;IGF Ⅰ组胰岛Fas的表达少于对照组 (P <0 .0 1) ;FasL的表达多于对照组 (P <0 .0 1)。结论 早期应用IGF Ⅰ腹腔注射可以减少NOD鼠的胰岛炎症和糖尿病发生 ,其机制与Fas FasL介导的胰岛B细胞凋亡有关。
Objective To observe the incidence of insulitis and diabetes mellitus (NO) and its effect on the expression of Fas FasL in pancreatic islets by intraperitoneal injection of insulin-like growth factor Ⅰ (IGF Ⅰ). Methods 20 0 4-week-old NOD female rats were randomly divided into 2 groups with 10 rats in each group. The experimental group was intraperitoneally injected with 1 mlIGF Ⅰ (40ng ml) twice a week and the control group with 1 ml normal saline for 8 weeks. The experimental animals were sacrificed 7 days after onset of diabetes or 20 weeks of age. Serum insulin and C-peptide concentrations were observed. The islet HE staining and immunohistochemical staining were used to detect the expression of Fas FasL in islets. Results IGF Ⅰ significantly reduced the incidence of insulitis and diabetes in NOD mice (P <0.01). The expression of Fas in islet in IGF Ⅰ group was less than that in control group (P <0.01) (P <0. 01). Conclusion The early application of IGF Ⅰ intraperitoneal injection can reduce NOD mice islet inflammation and diabetes mellitus, the mechanism and Fas FasL mediated islet B cell apoptosis related.