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目的观察常压氧疗(NBO)持续不同时间对大鼠脑缺血再灌注损伤的影响。方法将70只健康雄性SD大鼠随机分为假手术组,缺血再灌注组,NBO 3h组、6h组、9h组、12h组、24h组各10只。假手术组只结扎颈总动脉和颈外动脉,其余各组在此基础上采用线栓法建立大鼠脑缺血再灌注模型,NBO各组分别采用NBO持续3h、6h、9h、12h、24h。再灌注24h时进行神经功能缺陷评分,测定脑梗死体积,检测缺血侧脑组织超氧化物歧化酶活性和丙二醛、肿瘤坏死因子-α含量。结果与缺血再灌注组相比,NBO 3h、6h组神经功能缺陷评分和肿瘤坏死因子-α含量显著减低(均P<0.05),NBO 3h、6h、9h组脑梗死体积显著降低(均P<0.05),持续6h尤为明显;NBO 3h组超氧化物歧化酶活性显著升高,丙二醛含量显著降低(均P<0.05),NBO 6h组超氧化物歧化酶活性和丙二醛含量无显著性差异(均P>0.05),而NBO持续9～24h超氧化物歧化酶活性显著降低,丙二醛含量显著增加(均P<0.05)。结论NBO持续3～6h对大鼠脑缺血再灌注损伤有神经保护作用,能减小梗死体积,不增加氧化应激和炎症反应。
Objective To observe the effects of normobaric oxygen therapy (NBO) for different periods on cerebral ischemia-reperfusion injury in rats. Methods Seventy healthy male SD rats were randomly divided into sham operation group, ischemia reperfusion group, NBO 3h group, 6h group, 9h group, 12h group and 24h group. The rats in sham-operated group were given only carotid artery and external carotid artery. The rats in each group were subjected to NBO for 3h, 6h, 9h, 12h, 24h . Neurological deficit scores were measured at 24h after reperfusion. The volume of cerebral infarction was measured. The activity of superoxide dismutase (SOD), malondialdehyde (MDA) and tumor necrosis factor-α (TNF-α) Results Compared with ischemia-reperfusion group, NBO 3h, 6h group had significantly lower score of neurologic deficit and tumor necrosis factor-α (all P <0.05), and volume of cerebral infarction significantly decreased in NBO 3h, 6h, 9h group <0.05), especially for 6h. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in NBO 3h group were significantly lower than those in NBO 6h group (P <0.05) (P> 0.05). However, the activities of superoxide dismutase (SOD) in NBO decreased significantly and the content of malondialdehyde (MDA) increased significantly (P <0.05). Conclusion NBO sustained neurotoxicity after 3 ~ 6 h of cerebral ischemia-reperfusion injury can reduce the infarct volume, but not increase the oxidative stress and inflammatory reaction.