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目的探讨大鼠下颌下腺组织中是否表达卵泡刺激素(FSH)及其受体(FSHR),为进一步研究FSH对下颌下腺的功能调节提供理论依据。方法正常SD大鼠20只,体质量(200±20)g,腹腔麻醉后切取下颌下腺,采用原位杂交方法对FSH及FSHR进行细胞定位,探讨大鼠下颌下腺是否存在FSH及其受体mRNA,从下颌下腺组织中分别提取总RNA,运用RT-PCR获得FSH及其受体基因的cDNA核心序列,并对其序列进行分析。结果大鼠下颌下腺浆液性腺泡及颗粒曲管上皮细胞含有FSH和FSHR mRNA杂交信号,信号物质分布于胞质内,胞核呈阴性反应。从大鼠下颌下腺组织中扩增的FSH及FSHR基因的特异性条带分别为193bp和413bp。结论大鼠下颌下腺浆液性腺泡及颗粒曲管上皮细胞能合成卵泡刺激素及其受体,进一步说明下颌下腺是FSH作用的靶器官。
Objective To investigate whether FSH and FSHR are expressed in submandibular gland tissue of rats and provide a theoretical basis for further study on the functional regulation of submandibular gland by FSH. Methods Twenty normal SD rats (200 ± 20 g) were given intraperitoneal anesthesia. The submandibular glands were excised and the FSH and FSHR were localized by in situ hybridization. The expression of FSH and its receptor mRNA The total RNA was extracted from the submandibular gland tissue. The cDNA core sequence of FSH and its receptor gene was obtained by RT-PCR. The sequence of the cDNA was analyzed. Results The rat submandibular gland serous acinar and granular tubule epithelial cells contained FSH and FSHR mRNA hybridization signals. The signal substances distributed in the cytoplasm and the nuclei were negative. Specific bands of FSH and FSHR genes amplified from rat submandibular glands were 193 bp and 413 bp, respectively. Conclusion The submandibular gland serous acinar and granulovascular epithelial cells can synthesize follicle stimulating hormone and its receptor, further illustrating that submandibular gland is the target organ of FSH.