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目的:探讨不同保存方法对脑胶质瘤和其周边组织RNA保存的影响,为脑胶质瘤组织库规范化建设提供理论依据。方法:选择复旦大学附属华山医院脑胶质瘤组织库中采用新(RNALater法组5组)、旧(速冻法组4组)两种方法保存的脑胶质瘤组织共9组27份样本(包含肿瘤组织及其配对的瘤周组织),分别抽提总RNA,测定其A_(260/280)比值,凝胶电泳判断其完整性,比较不同保存方法对脑胶质瘤和其周边组织RNA保存的影响。结果:RNA质检合格率RNALater法组为100%,速冻法组为33.3%,两者差异有统计学意义(P<0.05)。质检结果为速冻法组中3份肿瘤组织合格,1份近瘤周组织合格,无远瘤周组织合格。结论:RNALater在保存RNA、预防RNA降解方面优于速冻法组。
Objective: To investigate the effect of different preservation methods on the RNA preservation of glioma and its surrounding tissues, and to provide a theoretical basis for the standardization of glioma tissue bank. Methods: A total of 27 glioma tissue samples were collected from the brain glioma tissue bank of Huashan Hospital affiliated to Fudan University. The glioma tissues of the glioma tissues of the new group (5 groups in the RNALater group) and the old group (4 groups in the quick-freezing group) Including tumor tissue and its matched peritumoral tissues). The total RNA was extracted and the A_ (260/280) ratio was determined. The integrity of the tumor was evaluated by gel electrophoresis. The effects of different preservation methods on the expression of glioma and its surrounding tissue RNA The impact of saving. Results: The qualified rate of RNA was 100% in RNALater group and 33.3% in quick freezing group, the difference was statistically significant (P <0.05). Quality inspection results for the quick-frozen method in the three groups of qualified tumor tissue, a tumor near the peritumoral organization, no peritumor tissue pass. Conclusion: RNALater is superior to rapid freezing method in RNA preservation and RNAi prevention.