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目的 :研究巨噬细胞炎性蛋白 - 1α(MIP - 1α)、调节激活正常T细胞表达和分泌细胞因子 (RANTES)基因和蛋白在哮喘小鼠支气管中的表达情况。方法 :2 0只二级雄性BALB/C小鼠随机分为两组 :正常小鼠组 (A0 组 )和哮喘小鼠组 (B0 组 )。以卵清白蛋白 (OVA)致敏激发法复制小鼠哮喘模型 ,采用免疫组化法和原位杂交法测定两组小鼠支气管MIP - 1α、RANTES蛋白及基因的表达情况。结果 :免疫组化显示 ,B0 组支气管MIP - 1α、RANTES蛋白的表达均显著高于A0 组 (P <0 0 1) ,其主要表达细胞是上皮细胞 ;原位杂交亦显示 ,B0 组支气管MIP - 1α、RANTES基因的表达均显著高于A0 组 (P <0 0 1) ,其主要表达细胞是上皮细胞。结论 :本研究证明MIP - 1α和RANTES等在实验性哮喘时支气管中的高表达 ,上皮细胞是主要表达细胞。
AIM: To investigate the expression of macrophage inflammatory protein - 1α (MIP - 1α), the expression of activated normal T cells and the expression of RANTES gene and protein in the bronchus of asthmatic mice. Methods: Twenty male BALB / C mice were randomly divided into two groups: normal mice (A0) and asthmatic mice (B0). Mouse asthma model was induced by ovalbumin (OVA) sensitization, and the expressions of MIP - 1α, RANTES protein and gene in bronchus were detected by immunohistochemistry and in situ hybridization. Results: Immunohistochemistry showed that the expression of MIP - 1α and RANTES protein in bronchus of B0 group was significantly higher than that in A0 group (P <0.01), and the expression of MIP - 1α and RANTES in epithelial cells was mainly detected by immunohistochemistry. In situ hybridization also showed that bronchial MIP - 1α, RANTES gene expression were significantly higher than the A0 group (P <0.01), the main cells are epithelial cells. Conclusion: This study demonstrates that MIP - 1α and RANTES are highly expressed in the bronchus in experimental asthma. Epithelial cells are the major expressed cells.