释放质子的酶反应常通过滴加碱溶液将反应控制在恒pH条件下进行研究。由于碱溶液的加入,随着反应的进行,反应体系的体积不断增加。本文研究了这种变体积的酶反应动力学,并用青霉素酰胺酶水解苄青霉素的反应进行了验证。 The proton-releasing enzyme reaction is often studied by controlling the reaction under constant pH by dropping an alkaline solution. Due to the addition of alkali solution, as the reaction progresses, the volume of the reaction system increases. In this paper, we studied the kinetics of enzyme reaction with this variable volume and validated the hydrolysis of benzyl penicillin with penicillin amidase.