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目的研究一株海洋细菌M44的化学成分。方法采用ODS C18反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及高效液相色谱等分离手段对发酵得到的乙酸乙酯相进行分离、纯化;利用理化性质和波谱学分析对单体化合物进行结构鉴定并用MTT法评价其体外细胞毒活性。结果从海洋细菌Sulfitobacter sp.(M44)发酵物中分离得到6个环二肽类化合物,分别鉴定为Cyclo-(L-Leu-D-val)(1)、Cyclo-(L-Phe-D-val)(2)、Cyclo-(L-Phe-L-Leu)(3)、Cyclo-(L-Leu-L-Ile)(4)、Cyclo-(D-Phe-L-Ile)(5)、Cyclo-(L-Trp-L-Pro)(6)。体外活性实验表明:化合物2,4对HepG2细胞株均表现出一定的生长抑制活性,其IC50分别为88μg/mL和105μg/mL,其中化合物4还对Hep2细胞株表现出生长抑制活性,其IC50为115μg/mL。结论以上化合物均为首次从该属细菌的代谢产物中分离得到,为进一步开发利用东海微生物资源奠定了一定的基础。
Objective To study the chemical composition of a marine bacterium, M44. Methods The ethyl acetate phase was separated and purified by ODS C18 reverse phase silica gel column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid chromatography (HPLC). The physicochemical properties and spectral analysis of the monomer compounds Structure identification and evaluation by MTT cytotoxic activity in vitro. Results Six cyclic dipeptides were isolated from marine bacteria Sulfitobacter sp. (M44) and identified as Cyclo- (L-Leu-D-val) (1) Cyclo- (L-Phe-L-Leu) (3), Cyclo- (L-Leu-L-Ile) , Cyclo- (L-Trp-L-Pro) (6). In vitro activity experiments showed that compounds 2 and 4 showed certain growth inhibitory activity on HepG2 cells with IC50 of 88μg / mL and 105μg / mL, respectively. Compound 4 also showed a growth inhibitory activity on Hep2 cells with IC50 Was 115 μg / mL. CONCLUSION The above compounds were isolated from the metabolites of the genus for the first time, which laid a solid foundation for the further development and utilization of microbial resources in the East China Sea.