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本研究通过卷丹百合在不同浓度的盐胁迫下确定了盐浓度阈值,利用同源克隆和RACE技术获得了盐阈值浓度处理下卷丹百合MAPK基因的保守区(Genbank登录号:JQ437268),并对其进行生物信息学分析。同时利用了DNAman软件和Blastn序列比对在基因和蛋白角度分析了MAPK基因在盐阈值浓度处理下的表达情况。分析表明,卷丹百合盐阈值浓度为1.09 mg/m L,MAPK基因保守区长627 bp,推测编码209个氨基酸,有1个高度保守的区域(TRWYRAPE)存在于MAPK氨基酸序列中,通过NCBI上的BLAST比对分析,发现这段保守区序列为促分裂原活化蛋白激酶超家族的催化活性区。
In this study, the salt concentration threshold was determined under different concentrations of salt stress with Lilium regia, and the conserved region (accession number: JQ437268) of MAPK gene was prepared by homologous cloning and RACE techniques under salt threshold concentration Bioinformatics analysis. At the same time, the DNAman software and Blastn sequence alignment were used to analyze the expression of MAPK gene under the threshold salt concentration in terms of gene and protein. The results showed that the threshold concentration of Lilium lilium was 1.09 mg / m L, the conserved region of MAPK gene was 627 bp, and deduced a polypeptide of 209 amino acids. One highly conserved region (TRWYRAPE) existed in the amino acid sequence of MAPK. BLAST analysis showed that this conserved region was found to be the catalytically active region of the mitogen-activated protein kinase superfamily.