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目的：探讨 Ｃ Ｅ Ａ重组痘苗病毒对 Ｃ Ｅ Ａ 阳性肿瘤的预防作用。方法：构建 Ｃ Ｅ Ａ 阳性小鼠 Ｈｅｐａ 肝癌细胞模型（ Ｈｅｐａ Ｃ Ｅ Ａ＋ ） 。将实验鼠分为４ 组，先分别皮下接种痘苗病毒（ 野生型 Ｗ Ｖ Ｖ 或重组型 Ｃ Ｅ Ａｒ Ｖ）３ 次，再分别颈背部皮下注射 Ｃ Ｅ Ａ 阳性或 Ｃ Ｅ Ａ 阴性同源鼠 Ｈｅｐａ 肝癌细胞。观察动物反应并在接种癌细胞后每周测量一次肿瘤大小。结果：３ 个对照组各鼠颈背部皮下均可见有瘤块形成，并且以相近速率快速增长，３ 组之间无统计学上的显著差异，而接种 Ｃ Ｅ Ａｒ Ｖ／ Ｈｅｐａ Ｃ Ｅ Ａ＋ 组各鼠在观察期限内均无肿瘤形成。整个实验过程中实验鼠未表现有毒副反应。结论： Ｃ Ｅ Ａｒ Ｖ 通过诱导机体的免疫系统产生 Ｃ Ｅ Ａ 特异性的细胞和体液免疫应答反应，能有效预防小鼠移植性 Ｃ Ｅ Ａ 阳性肿瘤的形成。
Objective: To investigate the preventive effect of C E A-recombinant vaccinia virus on C E A positive tumors. METHODS: A HEA-positive hepatocellular carcinoma cell model (HepaC E A+) was constructed. Rats were divided into 4 groups and subcutaneously inoculated with vaccinia virus (wild type W V V or recombinant C E A r V ) three times subcutaneously and then injected respectively into the back of the neck with C E A positive or C E A negative. Source Hepa liver cancer cells. Animal responses were observed and tumor size was measured once weekly after inoculation of cancer cells. RESULTS: In the three control groups, tumors were found subcutaneously on the back of the neck of each rat, and they grew rapidly at similar rates. There was no statistically significant difference between the three groups. Inoculation with C E A r V/ Hepa C E None of the rats in the A+ group developed tumors during the observation period. Rats did not show toxic side effects throughout the experiment. Conclusion: C E A r V can induce C E A specific cellular and humoral immune responses by inducing the body’s immune system, which can effectively prevent the formation of transplanted C E A positive tumors in mice.