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目的探讨大鼠趋化素样因子(Cklf1)在体外对血管平滑肌细胞增殖活性的影响。方法将pCDB／Cklf1真核表达载体(实验组)和空载体pCDB(对照组)分别转染COS-7细胞,获取上清液,刺激原代培养的大鼠主动脉血管平滑肌细胞,以噻唑蓝(MTT)法检测细胞增殖情况,并比较在光镜和电镜下形态学的改变。结果以10％胎牛血清稀释的实验组上清与对照组相比,MTT 法检测的A值在刺激大鼠平滑肌细胞48和72 h后差异有统计学意义(P<0．05和P<0．01,n= 5)。两组细胞光镜下形态学无明显差异,电镜下经Cklf1刺激后的平滑肌细胞亚细胞器及肌丝和致密体增多。结论 Cklf1在血管平滑肌的增殖过程中发挥重要作用。
Objective To investigate the effects of rat chemokine-like factor (Cklf1) on the proliferation of vascular smooth muscle cells in vitro. Methods The pCDB / Cklf1 eukaryotic expression vector (experimental group) and empty vector pCDB (control group) were transfected into COS-7 cells respectively, and the supernatant was obtained to stimulate primary cultured rat aortic vascular smooth muscle cells. (MTT) method to detect cell proliferation, and to compare the changes of morphology under light and electron microscopy. Results Compared with the control group, the supernatant of experimental group diluted with 10% fetal bovine serum showed a significant difference (P <0.05 and P < 0.01, n = 5). Morphology of the two groups of cells under light microscopy showed no significant difference. Electron microscope increased the number of subcellular organelles and myofilaments and dense bodies of smooth muscle cells stimulated by Cklf1. Conclusion Cklf1 plays an important role in the proliferation of vascular smooth muscle.