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以玻璃为基材的微流控芯片具有光学透明、亲水性好和强度高的特点.介绍了一种具有可更换丝状电极的全玻璃微流控芯片流通检测池.该芯片通过玻璃光刻、湿法刻蚀和热封合制备.一对Pt丝电极通过平行的脊突精确定位在检测通道的两侧,之间相距190 μm,流通检测池功能区的总体积仅约为300 nL.包括电化学(EC)、电化学发光(ECL)及化学发光(CL)的多模式检测均在此池体上进行了成功展示.利用微通道中的层流效应实现了电极之间的有效隔离.该芯片流通检测池具有耐用、透明、死体积小和与微型化在线流动检测相匹配的特点,可在高灵敏流动生物传感检测中得到应用.“,”Microfluidic chips based on the glass substrate are characterized by optical transparency,good hydrophilic and high strength.A microfluidic chip flow detection cell based on the total glass substrate with replaceable wire electrodes was introduced.The chip cell was prepared by photolithography,wet etching and thermal bonding of the glass substrate.A pair of Pt wire electrodes were precisely positioned parallelly along the two sides of the main microchannel and 190 μm apart from each other by the parallel ridges formed during the wet etching.The total volume of the functional part of the channel is only about 300 nL.Multiple detections including electrochemistry (EC),electrochemilluminescence (ECL) and chemilluminescence (CL) were successfully demonstrated with the chip cell.The effective isolation of the electrodes was achieved by the laminar flow effect in the microchannel.The chip flow detection cell is characterized in robustness,transparency,small dead volume and being compatible with the miniaturized online flow detection,thus can be used for the detection with high sensitive flow biosensors.