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应用原位分子杂交技术及细胞图象分析证实了人肝癌细胞株ＳＭＭＣ７７２１细胞经１０μｇ／ｍｌ的ＧＭ_３／ＧＤ_３处理后，ＧＤ_３处理组细胞内Ｎ－ｒａｓ基因ｍＲＮＡ的阳性信号弥漫分布于整个细胞，而经ＧＭ_３处理组和对照组阳性信号集中于核膜。ＧＭ_３／ＧＤ_３处理组及对照组ｃ－ｆｏｓ基因ｍＲＮＡ的阳性信号均集中于核膜，但ＧＭ_３处理组信号强度最大。上述结果提示，ＧＤ_３可使某些肿瘤细胞向更加恶性的方向发展，而ＧＭ_３则可使某些肿瘤细胞向正常方向转化。
The in situ molecular hybridization technique and cell image analysis confirmed that after treatment of human hepatocellular carcinoma cell line SMMC7721 with 10 μg/ml GM_3/GD_3, the positive signal of N-ras mRNA in the GD_3 treated group diffusely distributed throughout the cell. Positive signals were concentrated in the nuclear membrane in the GM3 treated and control groups. The positive signals of c-fos mRNA in GM_3/GD_3 treatment group and control group were all concentrated in the nuclear membrane, but the signal intensity of GM_3 treatment group was the highest. The above results suggest that GD_3 can make certain tumor cells develop in a more malignant direction, while GM_3 can make certain tumor cells transform in a normal direction.