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目的研究特发性中枢性性早熟(ICPP)女性患儿血浆中Kisspeptin及骨钙素(BGP)水平,并探讨其对ICPP女童诊断及疗效评估的意义。方法选取ICPP女童20例(ICPP组)、单纯乳房发育女童18例(PT组)及同期健康体检的健康未发育女童22例(对照组),采集ICPP组女童治疗前、治疗12个月后及PT组和对照组女童抗凝静脉血,采用酶联免疫吸附法(ELISA)检测其血浆Kisspeptin及BGP水平。结果治疗前ICPP组血浆Kisspeptin及BGP水平为(1.75±0.11)μg/L和(0.54±0.08)μg/L,明显高于PT组的(1.39±0.12)μg/L和(0.22±0.08)μg/L及对照组的(1.37±0.10)μg/L和(0.20±0.07)μg/L,差异均有统计学意义(P<0.01);PT组与对照组比较差异无统计学意义(P>0.05)。ICPP治疗12个月后的女童血浆Kisspeptin及BGP水平为(1.49±0.15)μg/L和(0.23±0.05)μg/L较治疗前明显降低,差异有统计学意义(P<0.01);与PT组及对照组比较差异均无统计学意义(P>0.05)。结论血Kisspeptin及BGP水平对诊断ICPP及疗效评估具有重要的参考价值。
Objective To study the plasma levels of kisspeptin and osteocalcin (BGP) in children with idiopathic central precocious puberty (ICPP) and to evaluate the significance of their diagnosis and evaluation of ICPP girls. Methods Twenty girls (ICPP group), 18 girls (PT group) and healthy uncoated girls (22 girls) were enrolled in this study. The girls of ICPP group were collected before treatment and 12 months after treatment The anticoagulant venous blood was collected from PT group and control group. The levels of Kisspeptin and BGP in plasma were detected by enzyme-linked immunosorbent assay (ELISA). Results The plasma levels of Kisspeptin and BGP in ICPP group before treatment were (1.75 ± 0.11) μg / L and (0.54 ± 0.08) μg / L, respectively, significantly higher than those in PT group (1.39 ± 0.12 and 0.22 ± 0.08) μg / L and (1.37 ± 0.10) μg / L and (0.20 ± 0.07) μg / L respectively in the control group (P <0.01). There was no significant difference between the PT group and the control group (P> 0.05). The plasma levels of Kisspeptin and BGP in girls treated with ICPP for 12 months were (1.49 ± 0.15) μg / L and (0.23 ± 0.05) μg / L, respectively, which were significantly lower than those before treatment (P <0.01) There was no significant difference between the two groups (P> 0.05). Conclusion The serum levels of Kisspeptin and BGP have important reference value for the diagnosis of ICPP and evaluation of curative effect.