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目的:建立黑鳗藤药材中黑鳗藤新苷K的含量测定方法。方法:应用反相高效液相色谱法测定黑鳗藤中黑鳗藤新苷K的含量,色谱柱使用D iamonsil C-18(250mm×4.6mm,5μm);流动相为乙腈:水(48∶52);检测波长为355nm;流速1mL/m in。结果:黑鳗藤新苷K在进样量2.024~14.168 mg范围内线性关系良好,回归方程为:Y=144701X-9335.5,r=0.9998;平均回收率为98.69%,RSD为1.48%(n=9)。结论:该方法简便,灵敏,准确,重现性好,可用于黑鳗藤药材的含量测定和质量控制。
Objective: To establish a method for determining the content of glycoside K in black vinegar. Methods: The content of glycoside K was determined by RP-HPLC. The chromatographic column was Diamonsil C-18 (250mm×4.6mm, 5μm). The mobile phase was acetonitrile:water (48∶ 52); detection wavelength is 355 nm; flow rate is 1 mL/min. RESULTS: The linear relationship was good between 2.024 and 14.168 mg injections. The regression equation was: Y=144701X-9335.5, r=0.9998. The average recovery was 98.69% and the RSD was 1.48% (n= 9). Conclusion: The method is simple, sensitive, accurate and reproducible. It can be used for the determination and quality control of Radix.