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采用以CO2 为流动相的超临界流体色谱方法 ,以含 0 0 5 % (体积分数 )三乙胺的乙醇作为改性剂 ,对具有重要生物功能的大豆磷脂组成进行分析 ,获得了大豆磷脂提取物中 6个重要组分的定性结果 ,并讨论了流动相组成、操作温度和压力对分离的影响。对其中有代表意义的磷脂酰胆碱 (PC)进行了外标法定量分析 ,在PC质量浓度为0 0 2 0 g/L～ 0 0 75 g/L时具有较好的线性关系 ,PC加样回收率为 96 7% (n =5 ) ,重现性好。此方法可用于实际样品的分析
The composition of soy lecithin with important biological function was analyzed by using supercritical fluid chromatography with CO2 as mobile phase and ethanol containing 0 0 5% (volume fraction) triethylamine as the modifier. The qualitative results of six important components of the material were discussed. The effects of mobile phase composition, operating temperature and pressure on the separation were also discussed. The representative of the significance of phosphatidylcholine (PC) by external standard quantitative analysis of the PC mass concentration of 0 0 2 0 g / L ~ 0 0 75 g / L has a good linear relationship, PC plus The sample recovery was 96 7% (n = 5) with good reproducibility. This method can be used for the analysis of actual samples