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目的探讨不同骨密度(bone mineral density,BMD)骨质疏松模型小鼠基因表达谱的差异,分析骨质疏松基因表达谱系的特征。方法采用30只C57BL/6J去势雌性小鼠建立骨质疏松模型,以微计算机断层扫描(Micro-CT)技术测量其BMD。根据BMD选取10只小鼠并分为2组:BMD最高的5只小鼠分入H组(high BMD组),BMD最低的5只小鼠分入L组(low BMD组)。应用基因芯片技术检测两组骨质疏松小鼠基因的表达,通过Gene Ontology(GO)数据库对差异表达基因进行基因信息学分析。结果共筛选出差异表达基因684条,包含功能明确的基因610条,其中299条表达上调、311条表达下调。结论炎症以及免疫反应、细胞黏附、胶原代谢及骨质矿化等相关基因群的差异表达,可能对骨质疏松小鼠BMD的改变发挥重要作用。
Objective To investigate the difference of gene expression profiles in osteoporosis model mice with different bone mineral density (BMD) and to analyze the characteristics of osteoporosis gene expression profile. Methods 30 C57BL / 6J ovariectomized female mice were used to establish the model of osteoporosis. The BMD was measured by Micro-computed tomography (Micro-CT) technique. Ten mice were selected according to BMD and divided into two groups: the five mice with the highest BMD were assigned to the high BMD group, and the five mice with the lowest BMD were assigned to the low BMD group. The gene expression of osteoporosis mice in two groups was detected by gene chip technology. The genes of differential expression were analyzed by Gene Ontology (GO) database. Results A total of 684 differentially expressed genes were screened, including 610 genes with definite functions, of which 299 were up-regulated and 311 were down-regulated. Conclusion The differential expression of inflammation, immune response, cell adhesion, collagen metabolism and bone mineralization may play an important role in the changes of BMD in osteoporotic mice.