在比较简陋的条件下制种,经常会遇到母种的管壁或培养基表面感染杂菌.笔者通过多次试验,用酒精棉球擦拭试管内壁,其接种成功率在90％以上。具体做法是:在接种针上缠一条用酒精浸过的棉花,通过酒精灯将燃烧着的棉花送入试管内自行熄灭后,反复擦拭管壁(但要注意不可接触培养基),擦遍管壁后抽出管外即可。接种按常规法进行。此法特别是在接二级种时效果更好。因为接二级苗种,种块需更大一些,这样很易使种块碰着试管壁而造成污染,采用此法后即使 In the more primitive conditions of seed production, often encounter the mother of the tube wall or medium surface infection of bacteria .Through several experiments, the author wipe the inner wall of the tube with alcohol cotton balls, the inoculation success rate of more than 90%. The specific approach is: wrapped in an inoculation needles wrapped in alcohol-impregnated cotton, the alcohol lamp will burn the cotton into the test tube to go out, repeatedly wipe the wall (but not to touch the media), rub the pipe Out of the wall after the tube can be. Vaccination according to conventional methods. This method is particularly effective in the second level. Because the next two seed, species need to be larger, it is easy to make the kind of test tubes touch the wall and cause pollution, even after using this method