论文部分内容阅读
梨胶锈菌(Cymnosporangium asiaticum Miyabe ex Yamada)的重寄生菌(Tuberculina vinosa Sacc.)在PDA、综合马铃薯和梨叶汁培养基上能生长、产孢,以PDA最好,Czapek和硝酸铵蔗糖上生长差且不产孢。菌丝生长和产孢的适温20~30℃,适宜pH6~9,荧光可促进产孢而黑光有抑制作用。对碳源的利用,菌丝生长和干重以甘露醇、葡萄糖等6种碳源为好,甘露醇最佳,淀粉最差;葡萄糖、蔗糖、果糖和甘露醇中均能产孢,以葡萄糖最好。对氮源的利用,菌丝生长及干重以硝酸钾、甘氨酸和蛋白胨为好;产孢以天冬酰胺、蛋白胨和硝酸钾为好,甘氨酸和谷氨酸不产孢。孢子萌发适温15~30℃,相对湿度65~100%均能萌发,在水滴中萌发率最高,低于50%不萌发;最适pH4~6,低于pH3和高于pH8不萌发;光照有利于孢子萌发。
Tuberculina vinosa Sacc. Of Cymnosporangium asiaticum Miyabe ex Yamada was able to grow, sporulate on PDA, potato and pear leaf juice medium, best on PDA, on Czapek and on ammonium nitrate sucrose Poor growth and no sporulation. Mycelial growth and sporulation temperature 20 ~ 30 ℃, suitable pH6 ~ 9, fluorescence can promote sporulation and black light inhibition. The utilization of carbon source, mycelium growth and dry weight of mannitol, glucose and other six kinds of carbon source is good, mannitol the best, the worst starch; glucose, sucrose, fructose and mannitol can spore to glucose the best. Utilization of nitrogen sources, mycelial growth and dry weight to potassium nitrate, glycine and peptone is better; spores to asparagine, peptone and potassium nitrate is good, glycine and glutamic acid spores. Spore germination suitable temperature 15 ~ 30 ℃, relative humidity 65 ~ 100% can germinate, the highest germination rate in water droplets, less than 50% does not germinate; optimum pH4 ~ 6, lower than pH3 and higher than pH8 does not germinate; Contribute to spore germination.