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[目的]了解海南省百岁人群中CETP基因I405V和TaqIB突变位点的多态性。[方法]收集海南省276例百岁老人全血DNA,利用AS-PCR结合测序的方法检测CETP基因I405V及TaqIB突变位点的多态性。[结果]I405V多态位点II、IV、VV3种基因型频率分别为28.6%、55%、16.3%,I和V的等位基因频率分布为56.2%和43.8%。TaqIB位点B1B1、B1B2、B2B23种基因型的发生率10.7%,73.6%,19.4%,B1和B2等位基因频率分布为47.3%和52.7%。[结论]海南百岁人群中CETP基因I405V和TaqIB多态性与已报道的海南汉族普通人群存在明显差异,可能与海南长寿密切相关。
[Objective] To investigate the polymorphisms of CETP gene I405V and TaqIB in centenarians of Hainan Province. [Methods] The whole blood DNA of 276 centenarians in Hainan Province was collected. The polymorphisms of C403V and TaqIB sites in CETP gene were detected by AS-PCR and sequencing. [Results] The frequencies of genotypes II, IV and VV of I405V polymorphism were 28.6%, 55% and 16.3%, respectively. The frequency distributions of I and V alleles were 56.2% and 43.8%. The genotype frequencies of B1B1, B1B2 and B2B23 in TaqIB loci were 10.7%, 73.6% and 19.4%, respectively. The frequencies of alleles B1 and B2 were 47.3% and 52.7%, respectively. [Conclusion] The CETP gene I405V and TaqIB polymorphisms in a hundred-year-old population of Hainan Province are significantly different from those reported in Hainan Han population and may be closely related to Hainan longevity.