论文部分内容阅读
目的采用乙醇注入法制备重组人生长激素(rhGH)脂质体,并建立包封率的测定方法。方法采用乙醇注入法制备rhGH脂质体,建立葡聚糖凝胶柱分离结合HPSEC法测定包封率;以包封率为指标,通过正交设计优化处方和工艺。结果最优处方为磷脂的用量20.8g/L,磷脂与胆固醇质量比为2∶1,磷酸盐缓冲液的浓度为0.02mol/L。按最佳处方及工艺制得的rhGH脂质体包封率为60.50%,平均粒径185.72nm。结论乙醇注入法制备工艺简单,易于掌握,优选得到的脂质体处方和制备工艺稳定可行。
Objective To prepare recombinant human growth hormone (rhGH) liposomes by ethanol injection and to establish a method for determination of entrapment efficiency. Methods The rhGH liposomes were prepared by ethanol injection, and the entrapment efficiency was determined by Sephadex LH - 20 column chromatography. The entrapment efficiency was used as an index to optimize the formulation and technology by orthogonal design. Results The optimal prescription was the dosage of phospholipid 20.8g / L, the mass ratio of phospholipid to cholesterol 2:1, the concentration of phosphate buffer 0.02mol / L. According to the best prescription and technology rhGH liposome encapsulation efficiency was 60.50%, the average particle size of 185.72nm. Conclusion The ethanol injection method is simple and easy to master. The liposome preparation and the preparation process are stable and feasible.