2型糖尿病(T2DM)作为代谢性疾病,严重威胁着人体健康。血管病变是T2DM的重要并发症,是T2DM致残和致死的首要原因。高密度脂蛋白(HDL)及其主要成分载脂蛋白A-I对血管内皮具有多重保护功能,与T2DM血管病变风险呈负相关,但在T2DM状态下这种保护功能受损。T2DM HDL保护功能受损影响血管内皮细胞代谢,可能导致血管并发症,其分子机制不明。在本研究中,我们基于NMR的代谢组学技术,通过提取临床T2DM患者及非糖尿病患者HDL,作用于人脐静脉内皮细 Type 2 diabetes (T2DM) as a metabolic disease, a serious threat to human health. Vascular disease is an important complication of T2DM, which is the leading cause of T2DM disability and death. High-density lipoprotein (HDL) and its major component, apolipoprotein A-I, have multiple protective functions on vascular endothelium and are inversely related to the risk of T2DM vascular lesions, but this protective function is impaired in T2DM. Impaired protection of T2DM HDL affects the metabolism of vascular endothelial cells, which may lead to vascular complications, the molecular mechanism is unknown. In this study, we used NMR-based metabonomics techniques to extract human umbilical vein endothelial cells (HDLs) from clinical T2DM patients and non-diabetic HDLs