论文部分内容阅读
[目的]探讨冠心病痰瘀互结证小鼠模型的建立和评价方法。[方法]小鼠分为空白组:10只C57BL/6J小鼠,喂养普通饲料6周。痰瘀互结证模型组:10只ApoE基因敲除小鼠,喂养高脂饲料6周,并于小鼠处死前7 d每日皮下注射异丙肾上腺素(10 mg/kg),空白组给予等量的生理盐水。实验中观察小鼠的一般状态,第6周末检测小动物超声和血清中血脂水平,共同评价模型的建立。[结果]与空白组比较,痰瘀互结模型组小鼠舒张末期室间隔厚度(IVSd)、收缩末期室间隔厚度(IVSs)、舒张末期左心室后壁厚度(LVPWd)、收缩末期左室后壁厚度(LVPWs)均明显增厚(P<0.01),呈心腔扩大的心肌肥厚特征,左室质量(LVM)明显增大(P<0.05或P<0.01)。小鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)明显升高(P<0.05或P<0.01)。[结论]ApoE基因敲除小鼠通过高脂饲料喂养结合异丙肾上腺素诱导可以建立冠心病痰瘀互结证小鼠模型,模型稳定,易操作,重复性好。
[Objective] To investigate the establishment and evaluation of mouse model of phlegm, blood stasis and cross-over syndrome in coronary heart disease. [Methods] The mice were divided into blank group: 10 C57BL / 6J mice fed normal feed for 6 weeks. Phlegm and blood stasis model group: 10 ApoE knockout mice were fed high-fat diet for 6 weeks and injected subcutaneously with isoproterenol (10 mg / kg) 7 days before the mice were sacrificed, The same amount of saline. In the experiment, the general state of the mice was observed. At the end of the 6th week, the ultrasounds of the small animals and the serum lipid levels were detected to establish the common evaluation model. [Results] Compared with the blank group, the changes of end-diastolic ventricular septal thickness (IVSd), end-systolic septal thickness (IVSs), end-diastolic left ventricular posterior wall thickness (LVPWd) (LVPWs) were significantly thicker (P <0.01), showing cardiac enlargement of myocardial hypertrophy, left ventricular mass (LVM) increased significantly (P <0.05 or P <0.01). Serum total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) were significantly increased (P <0.05 or P <0.01). [Conclusion] ApoE knockout mice can establish a mouse model of phlegm, blood stasis and cross - docking syndrome by feeding with high fat diet and isoproterenol. The model is stable, easy to operate and reproducible.