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目的 寻找可用于诊断猪囊尾蚴病或可诱发宿主保护性免疫力的特异抗原。方法 用免疫血清筛选猪囊尾蚴头节 λZAP c DNA文库 ,阳性克隆基因测序 ,并亚克隆入表达载体 p ET2 8b。对重组体进行诱导表达并对表达产物进行 SDS- PAGE和 Western blot分析。结果 从 c DNA文库中筛到 2个阳性克隆 Cy1和 Cy4。 Cy4片段在原核体系 (p ET2 8b)中表达 ,获得高产量的融合蛋白Cy4。结论 Cy1为编码猪囊尾蚴抗原 B的片段 ;Cy4为一种新的猪囊尾蚴特异抗原 ,其作为诊断抗原或疫苗的潜力值得进一步研究。
Objective To find specific antigens that can be used to diagnose cysticercosis or host protective immunity. Methods The cDNA of λZAP c DNA of Cysticercus cellulosae was screened by immune serum. The positive clones were sequenced and subcloned into the expression vector p ET2 8b. The recombinant was induced to express and the expressed product was analyzed by SDS-PAGE and Western blot. Results Two positive clones, Cy1 and Cy4, were screened from the c DNA library. The Cy4 fragment was expressed in the prokaryotic system (p ET2 8b) to obtain a high yield of the fusion protein Cy4. Conclusion Cy1 is a fragment encoding antigen of Cysticercus cellulosae B. Cy4 is a new antigen of cysticercus cellulosae. Its potential as a diagnostic antigen or vaccine deserves further study.