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目的:探讨组织中丙氨酸浓度与甲状腺乳头状癌(PTC)的关系。方法:收集2019年3至4月于郑州大学第一附属医院行甲状腺切除术且术后病理确诊为PTC的患者的组织标本30例，利用高通量代谢组学的方法测定癌组织与癌旁组织内的丙氨酸浓度，以中位数(四分位间距)表示，应用Wilcoxon test非参数检验分析癌组织与癌旁组织中丙氨酸浓度差异，利用Mann-Whitney n U检验分析多种临床特征与癌组织中丙氨酸浓度的关系，以n P<0.05为差异有统计学意义。n 结果:癌组织丙氨酸浓度[0.427(0.354～0.498) μg/L]与癌旁组织丙氨酸浓度[0.280(0.238～0.338) μg/L]的差异有统计学意义(Z＝-3.383，n P<0.05)。癌组织中丙氨酸浓度与临床病理特征之间的关系分析结果显示，年龄<45周岁组[0.469(0.408～0.583) μg/L]、中央区淋巴结转移组[0.496(0.430～0.593) μg/L]、尿碘超标组[0.482(0.416～0.582) μg/L]、肿瘤直径≥1 cm组[0.469(0.432～0.601) μg/L]及鼠类肉瘤滤过性病菌致癌同源体B1(BRAF)基因突变型组[0.433(0.381～0.554) μg/L]癌组织中测得的丙氨酸浓度分别比年龄≥45周岁组[0.402(0.337～0.425) μg/L]、中央区淋巴无转移组[0.405(0.314～0.429) μg/L]、尿碘未超标组[0.4136(0.297～0.460) μg/L]、肿瘤直径<1 cm组[0.414(0.319～0.488) μg/L]及BRAF基因野生型组[0.395(0.337～0.402) μg/L]癌组织中测得的丙氨酸浓度高，差异均有统计学意义(n P<0.05)。n 结论:丙氨酸可能在PTC的发生、发展及侵袭过程中起着重要作用。“,”Objective:To explore the relationship between alanine concentration in tissues and PTC.Methods:Collected 30 tissue samples from patients who underwent thyroidectomy at the First Affiliated Hospital of Zhengzhou University from March to April 2019 and were pathologically diagnosed with PTC after the operation. Using high-throughput metabolomics method to determine the concentration of alanine which expressed by median (interquartile range) in thyroid cancer tissues and corresponding adjacent thyroid tissues, application of Wilcoxon test non-parametric test to analyze the difference of alanine concentration in cancer tissues and adjacent thyroid tissues and using Mann-Whitney n U test to analyze the relationship between various clinical features and the concentration of alanine in cancer tissues, n P<0.05 is considered statistically significant.n Results:The difference in the concentration of alanine in cancer tissues [0.427 (0.354-0.498) μg/L] and adjacent thyroid tissues [0.280(0.238-0.338) μg/L] was statistically significant ( n Z=-3.383, n P<0.05), the analysis of the relationship between the concentration of alanine in cancer tissues and clinical features show that the alanine concentration measured in cancer tissues in the age<45 years old group [0.469 (0.408-0.583) μg/L], the central lymph node metastasis group [0.496 (0.430-0.593) μg/L], the urine iodine excess group [0.482 (0.416-0.582) μg/L], the tumor diameter ≥1 cm group [0.469 (0.432-0.601) μg/L], and the BRAF gene mutation group [0.433 (0.381-0.554) μg/L] were respectively higher than those in the age ≥45 years old group [0.402 (0.337-0.425) μg/L] and central lymph node non-metastasis group [0.405 (0.314-0.429) μg/L], urine iodine group [0.414 (0.297-0.460) μg/L], tumor diameter <1 cm group [0.414 (0.319-0.488) μg/L] and BRAF wild-type group [0.395 (0.337-0.402) μg/L], and the differences were statistically significant (n P<0.05).n Conclusion:Alanine may play an important role in the occurrence, development and invasion of PTC.