论文部分内容阅读
目的:比较成年健康小鼠脑室下区及室周器官BrdU标记细胞的数量及分布,探讨生理状态下室周器官的神经生发功能。方法:成年健康BALB/c小鼠,腹腔注射BrdU(50 mg/kg),连续3 d给药。4 d后用含4%多聚甲醛的磷酸缓冲液灌注动物,取脑制备石蜡切片,免疫组织化学染色。结果:侧脑室室下区可见大量BrdU标记细胞(33.70%±1.92%),在最后区、穹窿下器及脉络丛内BrdU标记细胞分别为(2.42%±0.38%),(2.78%±0.67%)和(1.12%±0.24%),连合下器未见BrdU标记细胞。侧脑室室下区BrdU标记细胞分别与室周器官BrdU标记细胞相比较均具有显著差异(P<0.05)。最后区及穹窿下器BrdU标记细胞的数量分别与脉络丛的BrdU标记细胞的数量相比均具有显著差异(P﹤0.05)。结论:室周器官存在少量的BrdU标记细胞,这表明生理状态下成年小鼠室周器官具有一定程度的神经生发功能;但室周器官神经生发功能比室下区的神经生发功能弱的多。
OBJECTIVE: To compare the number and distribution of BrdU labeled cells in the subventricular zone and periventricular organs of adult healthy mice and investigate the neurogenic activity of the periventricular organs under physiological conditions. Methods: Adult healthy BALB / c mice were injected intraperitoneally with BrdU (50 mg / kg) for 3 consecutive days. Four days later, the animals were perfused with 4% paraformaldehyde in phosphate buffered saline and paraffin sections were prepared and immunohistochemically stained. Results: A large number of BrdU labeled cells were found in the subventricular zone of the lateral ventricle (33.70% ± 1.92%). The number of BrdU labeled cells in the posterior fornix and choroid plexus were 2.42% ± 0.38%, 2.78% ± 0.67% ) And (1.12% ± 0.24%). No BrdU labeled cells were found in the co-openers. The BrdU labeled cells in the ventricular subventricular zone were significantly different from the BrdU labeled cells in the ventricular tissues (P <0.05). The numbers of BrdU-labeled cells in the final zone and the fornix were significantly different from those in the choroid plexus (P <0.05). Conclusion: There are a few BrdU labeled cells in the periventricular organs, which indicates that the periventricular organs of the adult mice have a certain degree of neurogenic activity under physiological conditions. However, the neurogenic activity of the periventricular organs is much weaker than that of the subventricular zone.