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目的:通过观察和比较星蒌承气汤和补阳还五汤对脑缺血大鼠海马神经元的保护作用,明确两方治疗脑缺血的时相性特点。方法:大鼠随机分为假手术组、模型组、尼莫地平组、星蒌承气汤和补阳还五汤组;线栓法制备大鼠大脑中动脉阻塞;大鼠给药剂量为尼莫地平10.8 mg.kg-1、星蒌承气汤5.0 g.kg-1、补阳还五汤13.0 g.kg-1,造模前4 d灌胃用药,脑缺血术后每天用药1次;大鼠分别于1,3,7 d进行神经功能评分后取脑,测定脑组织含水量和脑梗死面积,观察海马神经元密度和病理损伤。结果:模型组大鼠神经功能明显降低(18.00±0.00 vs 7.43±1.27,8.86±1.34,10.25±1.03)、脑组织含水量增多(71.00±1.33)%vs(79.85±1.73)%,(80.84±0.98)%,(82.86±1.15)%、脑梗死面积增大(0.00±0.00)%vs(16.73±1.13)%,(17.89±1.54)%,(19.32±1.21)%、海马神经元密度减小(153.57±15.62,118.71±9.98,79.38±6.02)、脑组织病理损伤加重;各用药组的神经功能、脑含水量、神经元密度和脑组织病理损伤均较模型组改善,尼莫地平组3 d和7 d、各中药组的脑梗死面积较模型组减小;与尼莫地平组比较,补阳还五汤各组的神经评分增高,7 d的脑含水量减少、病理损伤减轻,两方药组的海马神经元密度增大;补阳还五汤组7 d的神经功能(13.29±1.11)、脑含水量(77.94±2.00)%、海马神经元密度(124.29±11.64)、脑组织病理损伤均较星蒌承气汤改善明显。结论:星蒌承气汤和补阳还五汤对脑缺血海马神经元受损均具有保护作用,二者在缺血早期(1 d和3 d)的作用未见明显差异,但随着缺血时间的延长(7 d),补阳还五汤方显示出更为全面和显著的神经保护和功能修复作用。
OBJECTIVE: To observe and compare the protective effects of Xinglou Chengqi Decoction and Buyang Huanwu Decoction on neurons in hippocampus of rats with cerebral ischemia, and to clarify the characteristics of the phases of both phases in treating cerebral ischemia. Methods: The rats were randomly divided into sham-operation group, model group, nimodipine group, Xinglou Chengqi Decoction and Buyang Huanwu Decoction group. The middle cerebral artery occlusion Morindapine 10.8 mg.kg-1, Xinglou Chengqi Decoction 5.0 g.kg-1, Buyang Huanwu Decoction 13.0 g.kg-1, 4 days before model administration, medication 1 day after cerebral ischemia The rats were sacrificed on the 1st, 3rd, 7th day respectively for neurological function. Brain water content and cerebral infarction area were measured. The density and pathological changes of hippocampal neurons were observed. Results: The neurological function of the model group was significantly lower (18.00 ± 0.00 vs 7.43 ± 1.27, 8.86 ± 1.34, 10.25 ± 1.03), the water content of brain tissue increased (71.00 ± 1.33)% vs (79.85 ± 1.73)%, (80.84 ± (82.86 ± 1.15)% and (0.00 ± 0.00)% vs (16.73 ± 1.13)%, (17.89 ± 1.54)% and (19.32 ± 1.21)%, respectively (153.57 ± 15.62,118.71 ± 9.98, 79.38 ± 6.02). The pathological damage of brain tissue was aggravated. The neurological function, brain water content, neuron density and pathological damage of brain tissue in each treatment group were better than those in model group. Nimodipine group 3 Compared with the nimodipine group, the neurological score of each group of BYHWD increased, the brain water content decreased 7 days, and the pathological damage alleviated The density of hippocampal neurons increased in Fangmiyuanjian decoction group; the neurological function (13.29 ± 1.11), brain water content (77.94 ± 2.00)%, hippocampal neuron density (124.29 ± 11.64), brain tissue pathology Injury were better than Xinglou Chengqi Decoction significantly. Conclusion: Xinglou Chengqi Decoction and Buyang Huanwu Decoction have the protective effects on the neuronal damage of hippocampus after cerebral ischemia. There is no significant difference between the two groups in early ischemia (1 d and 3 d) Ischemic time extension (7 d), Buyanghuanwu Tang Fang showed more comprehensive and significant neuroprotection and functional repair.