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目的观察心衰患者心肌组织中钠氢交换体1(sodium-hydrogen exchanger 1,NHE1)的表达及其与血清胶原的相关性,探讨NHE1在心衰发生和发展中的作用及其与心肌纤维化的相互关系。方法设心衰组及正常对照组,心衰组按NYHA分级分成心功能Ⅰ级、Ⅱ级及Ⅲ级3个亚组。采用实时荧光定量聚合酶链反应(FQ-PCR)检测受试者心肌组织中NHE1 mRNA的表达水平,采用放射免疫分析法分别测定血清Ⅰ型前胶原羧基端肽Ⅰ(PⅠCP)和Ⅲ型前胶原氨基端肽(PⅢNP)含量。结果心衰组PⅠCP、PⅢNP含量较正常对照组明显升高,随着心功能的逐渐恶化,心衰组PⅠCP、PⅢNP含量呈上升趋势,各组间表达总体上有差异(P<0.05或P<0.01),PⅠCP、PⅢNP含量与NHE1 mRNA的△Ct值呈显著负相关。结论心衰患者心肌组织中存在NHE1 mRNA的高水平表达,血清PⅠCP、PⅢNP含量显著增加,提示NHE1可能在心衰的发生和发展过程中起着重要作用。
Objective To observe the expression of NHE1 and its correlation with serum collagen in heart failure patients and explore the role of NHE1 in the occurrence and development of heart failure and its relationship with myocardial fibrosis The relationship between. Methods Heart failure group and normal control group, heart failure group were divided into NYHA class Ⅰ, Ⅱ and Ⅲ subgroups. The expression of NHE1 mRNA in myocardium was detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR). Radioimmunoassay was used to detect the expression of serum type Ⅰ procollagen carboxyterminal peptide Ⅰ (PⅠCP) and type Ⅲ procollagen Amino-terminal peptide (PⅢNP) content. Results The levels of PⅠCP and PⅢNP in heart failure group were significantly higher than those in normal control group. The levels of PⅠCP and PⅢNP in heart failure group increased with the gradual deterioration of cardiac function, and there was a significant difference between the groups (P <0.05 or P < 0.01). The contents of PⅠCP and PⅢNP were negatively correlated with △ Ct of NHE1 mRNA. Conclusions High expression of NHE1 mRNA exists in myocardial tissue of patients with heart failure. The contents of PⅠCP and PⅢNP in serum are significantly increased, suggesting that NHE1 may play an important role in the occurrence and development of heart failure.