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１９９５和１９９６年分别在武昌和云南红花病株上获得两个黄瓜花叶病毒（ＣＭＶ）分离物ＣＭＶ—ＣＷ和ＣＭＶ—ＣＹ。ＣＭＶ—ＣＷ引起红花黄化花叶、严重矮化，而ＣＭＶ—ＣＹ仅引起红花轻花叶。在供试２３种植物中，两个分离物侵染１６种植物。两个分离物均能被桃蚜以非持久性方式传播，不通过红花种传；体外稳定性状分别为：致死温度５５～６０℃和５０～６０℃，稀释限点１０－４～１０－５和１０－３～１０－４，体外存活期３ｄ和４ｄ；病毒颗粒球状，直径分别为２７．２ｎｍ和２８．０ｎｍ；外壳蛋白分子量分别为２７５００和２８１００Ｄ。ＣＭＶ—ＣＷ和ＣＹ血清学性质和ＣＭＶ—ＣＡ相近。通过ＲＴ—ＰＣＲ扩增，获得ＣＭＶ—ＣＷ和ＣＭＶ—ＣＹ预期的５３４ｂｐ病毒外壳蛋白基因ｃＤＮＡ片断。株系研究表明，参试的ＣＭＶ—ＣＷ、ＣＭＶ—ＣＹ和其他２个ＣＭＶ中国分离物在温度敏感实验和血清学性质上与ＣＭＶ—Ｄ株系一致或相近，属于ＣＭＶ亚组Ｉ。依据在一组寄主植物上的反应，将４个参试ＣＭＶ分离物作了初步株系划分。
Two cucumber mosaic virus (CMV) isolates, CMV-CW and CMV-CY, were obtained from Wuchang and Yunnan safflower in 1995 and 1996, respectively. CMV-CW caused yellowing mosaic flowers, severe dwarfing, while CMV-CY caused only the safflower light mosaic. Of the 23 plants tested, two isolates inoculated 16 plants. Both isolates could be disseminated in a non-persistent way by Myzus persicae, not by safflower. The in vitro stability traits were: lethal temperature 55-60 ℃ and 50-60 ℃, dilution limit 10-4-10- 5 and 10-3 ~ 10-4, in vitro survival of 3d and 4d; virus particles spherical, diameter 27.2nm and 28.0nm respectively; coat protein molecular weight of 27500 and 28100D. The serological properties of CMV-CW and CY are similar to those of CMV-CA. The 534 bp viral coat protein gene cDNA fragment expected from CMV-CW and CMV-CY was obtained by RT-PCR amplification. The lineage studies showed that CMV-CW, CMV-CY and two other Chinese CMV isolates were consistent or similar to CMV-D strains in temperature-sensitive experiments and serological properties, belonging to CMV subgroup I. Based on the reactions on a group of host plants, four preliminary CMV isolates were divided.