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毛木耳Auricularia polytricha子实体500g经热水浸提、乙醇沉淀、Sevage法脱蛋白后,得到粗多糖3.94g,得率为0.79%。经小鼠S180载体实验证实,50mg/kg该粗多糖对肿瘤的抑制率达47.89%,呈极显著差异。利用离子交换柱对其进行分离,共得到三个部分,分别命名为APPⅠ、APPⅡ、APPⅢ。其中,APPⅡ对小鼠S180的抑制率达到56.36%,明显优于其他两部分。通过SephacrylS300凝胶过滤层析柱后,APPⅡ被进一步分离为A和B两个部分,APPⅡA的抑瘤率为53.61%,远高于APPⅡB,呈极显著差异。通过紫外光谱、红外光谱等方法对该单一多糖组分进行结构分析,表明APPⅡA为一相对分子质量约为110,000Da、可能同时具有α和β构象的杂多糖,主要有木糖和甘露糖组成,且纯度较高,含有硫酸基。
Auricularia polytricha fruiting body 500g by hot water extraction, ethanol precipitation, Sevage method deproteinized to obtain crude polysaccharide 3.94g, a yield of 0.79%. The mice S180 carrier experiments confirmed that the crude polysaccharides 50mg / kg inhibition rate of 47.89% of the tumor, showed a very significant difference. Using ion exchange column to separate them, a total of three parts, named APPⅠ, APPⅡ, APPⅢ. Among them, APP Ⅱ on mouse S180 inhibition rate of 56.36%, significantly better than the other two parts. After SephacrylS300 gel filtration chromatography, APPⅡwas further separated into two parts A and B. The inhibitory rate of APPⅡA was 53.61%, which was much higher than that of APPⅡB. The structure analysis of this single polysaccharide component by UV and IR spectroscopy showed that APPⅡA is a heteropolysaccharide with a relative molecular mass of about 110,000 Da, which may have both α and β conformations, mainly including xylose and mannose, And high purity, containing sulfate.