【摘 要】
：
大蚊属Tipula Linnaeus,1758是大蚊科中种类最多的属,目前其单系性尚未得到全面验证.此外,长角大蚊亚属Tipula (Sivatipula) Alexander,1964因其极长的触角以及独有的精子泵结构,明显不同于大蚊属其他亚属,使其亚属的分类地位存在争议.本研究基于COI序列对19个大蚊属物种及5个其他属物种进行了系统发育分析,并计算了物种间的遗传距离.研究结果表明:(1)邻接树(NJ)和最大似然树(ML)均显示长角大蚊亚属与大蚊属其他亚属未形成单系,大蚊属的单系性没有得到支持;(2)
【机 构】
：
安庆师范大学生命科学学院,皖西南生物多样性研究与生态保护安徽省重点实验室,安徽安庆246011;安庆师范大学图书馆,安徽安庆246011;四面山森林资源服务中心,重庆江津402296
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大蚊属Tipula Linnaeus,1758是大蚊科中种类最多的属,目前其单系性尚未得到全面验证.此外,长角大蚊亚属Tipula (Sivatipula) Alexander,1964因其极长的触角以及独有的精子泵结构,明显不同于大蚊属其他亚属,使其亚属的分类地位存在争议.本研究基于COI序列对19个大蚊属物种及5个其他属物种进行了系统发育分析,并计算了物种间的遗传距离.研究结果表明:(1)邻接树(NJ)和最大似然树(ML)均显示长角大蚊亚属与大蚊属其他亚属未形成单系,大蚊属的单系性没有得到支持;(2)基于遗传距离和系统发育分析并结合形态信息,结果显示长角大蚊亚属独立于大蚊属内其他亚属,应将其提升为属级分类单元.“,”The large genus Tipula Linnaeus,1758 contributes heavily to the biodiversity of the family Tipulidae.However,the monophyly of Tipula has not yet been verified.The subgenus Sivatipula Alexander,1964 is possibly the most confusing subgeneric taxon in the genus Tipula because of its members\' particularly long antenna and one-armed posterior immovable apodeme on semen pump,which makes its subgeneric position uncertain.In this research,the sequences of cytochrome oxidase I (COI) for 19 Tipula species and five taxa from other genera are analyzed.Considering the molecular evidence on genetic distance as well as phylogenetic analysis and morphological information,our results indicate that (1) the genus Tipula is not resolved as monophyletic in phylogeny based on neighbor joining (NJ) and maximum likelihood (ML) trees because the subgenus Sivatipula doesn\'t form a monophyletic clade with the remaining subgenera of Tipula;and (2) Sivatipula may deserve a generic status since it forms an independent phylogenetic line.
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